Microarray gene expression profiles of the nci 60 cell lines. In order to access nci at frederick staff information, you must first login using your nih account. The nci60 panel, originally assembled to screen for anticancer agents 68, has been profiled more extensively at the molecular level than any other set of cells in existence. Drug sensitivity in cancer cell lines is not tissuespecific. Global proteome analysis of the nci60 cell line panel. Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. Nci60 human tumor cell line screen service altogen labs. Molecular profiles, drug response, and chemical structures for the nci60 cell lines. The nci60, a panel of 60 diverse human cancer cell lines used by the developmental therapeutics program of the u. Certificate of analysismsds is available upon request with lot number. Database of crosscontaminated or misidentified cell lines iclac. Data analysis in accordance with the in vitro cell line screening project ivclsp for the dtp anticancer drug discovery program this method uses seven absorbance measurements zero, control growth and test growth in the presence of drug at the five concentration levels. The nci60 is a panel of 60 human cancer cell lines used by the developmental therapeutics program dtp of the u. Victorias machine learning notes persagen consulting.
Because many laboratories around the world rely on data from the nci60 cells, confirmation of their genetic identities represents an essential step in validating. We describe here a set of integromic studies in which we have profiled the 60 human cancer cell lines the nci 60 used by the national cancer institute to screen 100,000 chemical compounds over the last years. Molecular profiles and drug response for the nci60 cell lines. Nci60 panel group of 60 cancer cell lines that are maintained by the national cancer institute and widely used in basic research and drug discovery in 2001 it was discovered that the breast cancer cell line mcf7 and its drug resistant daughter cell line mcf7 adrr adriamycin resistant were unrelated. In the latent layer we also introduced a neuron responsible for growth inhibition percentage, which when negative indicates the reduction in the number of tumor cells after the treatment. Dna profiling analysis of endometrial and ovarian cell. The gi50 represents the negative log of the concentration that is required to inhibit the growth of a cell line by 50%. Dna profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Nci60 panel group of 60 cancer cell lines that are maintained by the national cancer institute and widely used in basic research and drug discovery in 2001 it was discovered that the breast cancer cell line mcf7 and its drug resistant daughter cell line mcf. Full text of real world drug discovery a chemists guide to biotech and pharmaceutical research robert m. The paper examined gene expression in 60 human cancer cell lines at the nci, including one rae was using mdamb435, the most widely. Further information regarding the ncbi60 panel can be found on the national cancer institute website. Among 43,225 compounds screened for activity against the nci 60 cell lines as of september 2012, 15,898 showed high dynamic range in their gi50 estimates across all cell lines. National cancer institute to screen 100,000 compounds plus natural products since 1990 710. Full release of endpoints calculated from concentration curves.
This panel has been used as a key tool to develop and screen anticancer drugs. Mistaken identity shakes two families dateline nbc newsmakers. The name of the families book is mistaken identity. The nci60 panel of 60 human cancer celllines of nine different tissues of origin has been extensively characterized in biological, molecular and pharmacological studies.
The nci60 human tumor cell line panel is used by a broad range of cancer investigators and by the nci developmental therapeutics program dtp to discover novel anticancer drugs. Isolated cells and cell lines, including the novel cell line hpmecst1. Compound profiling for anticancer activity using the nci. Authentication of african green monkey cell lines using human short tandem repeat markers. We wanted to understand the contributions of specific mechanisms of genomic instability to the etiology of cancers represented by the nci60. Learn more about oncoleads in vivo cell panel screening. Identity of original and late passage dami megakaryocytes with hel erythroleukemia cells shown by combined cytogenetics and dna fingerprinting. Roswell park memorial institute medium, commonly referred to as rpmi, is a form of medium used in cell culture and tissue culture. After more than 25 years of heavy use by researchers around the world, the us national cancer institute nci has decided to retire the nci60, its panel of 60 human cancer cell lines grown in. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.
The proteome profiles of all individual cell lines followed a conventional onedimensional page followed by ingel digestion and liquid. I obtained different mfi mean fluorescence intensity values in fl1 between control cells and treated cells for example, 1 400 000 a. Establishment and characterization of five cell lines derived from human malignant gliomas. When a panel of experts set new guidelines for cancer pain control, nci wanted to share. The nci 60 cancer cell line panel is a group of 60 human cancer cell lines used by the national cancer institute nci for the screening of compounds to detect potential anticancer activity.
The nci60 is a collection of tumor cell lines derived from a variety of human adult cancer tissue types and is commonly used for genetic analysis and screening of potential chemotherapeutic agents. Considering the widely used nci 60 panel, we assessed whether drugs for a particular cancer type display a higher efficacy in cell lines supposedly representing the respective cancer tissue. The nci 60 panel the tp53 website the tp53 web site. Jun 26, 2018 full release of endpoints calculated from concentration curves.
Since the nci60 panel has been used in the screening of thousands of compounds including fdaapproved and investigational drugs, it is a unique resource for testing the relationship of variants in genes to drug response. Compound profiling for anticancer activity using the nci60. Proteotypic features of nci60 cancer cells obtained by. Database of crosscontaminated or misidentified cell.
Jun 23, 2009 the nci60 is a panel of 60 human cancer cell lines used by the developmental therapeutics program dtp of the u. Proteotypic features of nci60 cancer cells obtained by swath. Database of crosscontaminated or misidentified cell lines amanda capesdavis and r. Benchmark performance in developed system against applicable in vivo animal models and known clinical performance.
Many health communication experts contributed to the revision of this book. We focused on three cancer types, namely, breast, colorectal and prostate cancer, represented by five, seven and two cell lines, respectively, in the nci 60. A sensitivity of cancerspecific drugs intended for breast cancer 41, colorectal cancer 24 and prostate cancer 38 across the nci60 panel covering 59 cancer cell lines derived from nine cancer tissues. While the publisher and author have used their best efforts in preparing this book, they make no representations or warranties with respect to the accuracy or completeness of the contents of this book and specifically disclaim any implied warranties of merchantability or fitness for a particular. The nci60 panel comprises 59 individual cancer cell lines derived from nine different tissues brain, blood and bone marrow, breast, colon, kidney, lung, ovary, prostate, and skin, which we analyzed using three proteomic approaches figures 1a and s1as1c. Molecular and quantitative animal genetics mafiadoc.
Those diagnosed at age 60 or older comprise the majority of cancer survivors. Nci at frederick staff login national cancer institute. The nci60 panel is the national cancer institutess collection of 60 human cancerous cell lines. Stanford nci60 cancer microarray project web supplement to the paper from ross d. Molecular profiles, drug response, and chemical structures for the nci 60 cell lines bioconductor version. Database of crosscontaminated or misidentified cell lines.
Mistaken identity of widely used esophageal adenocarcinoma cell line te7. Integromic analysis of the nci60 cancer cell lines ios. The nci 60 is a collection of tumor cell lines derived from a variety of human adult cancer tissue types and is commonly used for genetic analysis and screening of potential chemotherapeutic agents. The nci60 cancer cell line panel is a group of 60 human cancer cell lines used by the. From the very beginning we closely followed the experimental procedures recommended and established in the developmental therapeutics program of the nci, commonly known as the nci 60 panel. Expression of micrornas in the nci60 cancer celllines. Animal cell culture cell culture gel electrophoresis. Microarray gene expression profiles of the nci 60 cell lines description. Drug response tree drt of mek inhibitor selumetinib integration of proteotypes and other features. Karyotypic complexity of the nci60 drugscreening panel. We wanted to understand the contributions of specific mechanisms of genomic instability to the etiology of cancers represented by the nci 60. Represents a broader spectrum of therapeutic indications 17 vs.
The cell line list included in the altogen96 panel contains a majority of the same cell lines used in the nci 60 human cancer cell line screen panel and are well characterized in regards to their genotype and phenotype, including databases available to download rna expression data, protein expression data, epigenetics and dna sequencing. The cell line list included in the altogen96 panel contains a majority of the same cell lines used in the nci60 human cancer cell line screen panel and are well characterized in regards to their genotype and phenotype, including databases available to download rna expression data, protein expression data, epigenetics and dna sequencing. A description of the nci 60 assay and calculations can be found here. Additional lines evaluated for use in the screen and currently available are listed separately at the bottom of the page. A description of the nci60 assay and calculations can be found here.
The nci60 panel includes cancers of colorectal, renal, ovarian, prostate, lung, breast, and central nervous system origin, as. The output of the aae was used to screen 72 million compounds in pubchem and select candidate molecules with potential anticancer properties. That molecular characterization, a central subject of this journals spotlight on molecular profiling series 9 18, provided motivation for the present study. Isolated cells and cell lines, including the novel cell line hpmec st1. These 60 human tumour cell lines are derived from patients with leukaemia, melanoma, along with, lung, colon, central nervous system, ovarian, renal, breast and prostate cancers. Integromic analysis of the nci60 cancer cell lines ios press. Please note the links for more information on the snb19, u251, nci adrres, and mdamb435 cell lines.
To train the aae we used the nci60 cell line assay data for 6252 compounds profiled on mcf7 cell line. It has traditionally been used for growth of human lymphoid cells. The nci60, a panel of 60 diverse human cancer cell lines used by the developmental therapeutics. Rpmi 1640 has traditionally been used for the serumfree expansion of human lymphoid. Rutka jt, giblin jr, dougherty dy, liu hc, mcculloch jr, bell cw, stern rs, wilson cb, rosenblum ml. This is a list of the 60 human cancer cell lines used in the screen and maintained at nci frederick. Jul 15, 20 the fact that the nci 60 panel has been used to screen thousands of compounds provides a rich resource for testing the relationship of variants in genes to drug response. Benchmark performance in developed system against 2d e.
Roschke av, tonon g, gehlhaus ks, mctyre n, bussey kj, lababidi s, scudiero da, weinstein jn, kirsch ir. Understanding and managing cell culture contamination. Comprehensive list of cancerrelated genetic variations of. After more than 25 years of heavy use by researchers around the world, the us national cancer institute nci has decided to retire the nci 60, its panel of 60 human cancer cell lines grown in. Analyses of data from such studies have provided valuable information for understanding cellular processes and developing strategies for the diagnosis and treatment of cancer. Authentication of african green monkey cell lines using. A case of mistaken identity the scientist magazine. Consists of 6,830 gene expressions and 64 cancer cell lines. This panel represents an invaluable and publicly accessible platform of pharmacological, genomic, metabolomic, biochemical, and molecular datasets 38.
The book lists its authors as don and susie van ryn. To train the aae we used the nci 60 cell line assay data for 6252 compounds profiled on mcf7 cell line. The nci 60 panel is the national cancer institutess collection of 60 human cancerous cell lines. This medium contains a great deal of phosphate and is formulated for use in a 5% carbon dioxide atmosphere. Further information regarding the ncbi 60 panel can be found on the national cancer institute website. Microarray gene expression profiles of the nci 60 cell. Karyotypic complexity of the nci 60 drugscreening panel. The topics of molecular and quantitative animal genetics have been chosen to help students understand the concepts, principles, and models of animal molecular and quantitative genetics and to learn to apply these concepts for improving the health, production, and wellbeing of animals. Nci60 is a dataset of gene expression profiles of 60 national cancer institute nci cell lines.
Panel to appraise the national cancer program as established in accordance. Tissue specificity displayed by canerspecific drugs. Lorenzi pl, reinhold wc, varma s, hutchinson aa, pommier y, chanock sj, weinstein jn. Molecular profiles and drug response for the nci 60 cell lines. This is a list of the 60 human cancer cell lines used in the screen and maintained at ncifrederick. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies. Studies suggest anywhere from 1520% of the time, cells used in experiments have been misidentified or contaminated with another cell line. Two families, one survivor, unwavering hope by don. Developing and pretesting concepts, messages, and materials. From the very beginning we closely followed the experimental procedures recommended and established in the developmental therapeutics program of the nci, commonly known as the nci60 panel. Since the nci 60 panel has been used in the screening of thousands of compounds including fdaapproved and investigational drugs, it is a unique resource for testing the relationship of variants in genes to drug response. Nci60 growth inhibition data nci dtp data nci wiki. Mark is currently working with alec baldwin on his book, a promise to. We describe here a set of integromic studies in which we have profiled the 60 human cancer cell lines the nci60 used by the national cancer institute to screen 100,000 chemical compounds over the last years.
333 31 601 239 1560 1065 933 356 565 1015 1000 368 941 917 65 736 1502 1122 372 1394 780 1443 1069 1392 592 135 1092 21